Test 2. The Smoothness And Adsorption Evaluation Of 96-well Plate

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Aim
To compare the smoothness and adsorption of biousing 96-well plate with international brand C.

Materials and methods

Materials:

96-well paltes
biousing and brand C 96-well plates were tested in this experiment. 5 plates of biousing or brand C were tested in this experiment.

Instrument
ELIASA is from Beijing Pulang Novotech Co., LTD. Digital Height measuring instrument is from DIGIMAR, Germany.

Method:

Smoothness test

1. Data of smoothness on the upper surface

Digital Height measuring instrument was used to randomly measure the height 4 points of each well. The data was processed by statistic software and the tolerance was calculated.

2. Data of smoothness on the lower surface

Pictures were taken to show the smoothness of the lower surface.

Adsorption test

The plates were tested in ELIASA under 450-630nm wavelength. The data were processed by statistic software.

Thickness of the bottom

Digital Height measuring instrument was used to measure the bottom thickness of biousing, brandC and brand B.

The following pictures show the difference between the two brands in 96-well plates. The lower bottom of biousing plates is more even than that of other famous brands. This priority helps users to eliminate the refraction error in the experiment.

Results

Smoothness test:

1. Data of smoothness on the upper surface were in table 2.

Table 2 The comparison of smoothness on the upper surface between biousing and brand C of 96-well plates (384 points tested):

* Significant difference (P<0.05)

2. Data of smoothness on the lower surface

The following pictures show the difference between the two brands in 96-well plates.

biousing 96-well plate

Brand C 96-well plate

Adsorption test:

The lower bottom of biousing plates is smoother than that of brand C. The data of adsorption were in table 3.

Table 3 The contrast of adsorption between biousng and brand C 96-well plates:

* Significant difference (P<0.05)

Thickness of the bottom:

The bottom thickness of biousing, brandC and brand B were measured and the data are in table 4.

Table 4 the bottom thickness of biousing and other brands:

Conclusion

96-well plates are well used in many experiments. They can be either tissue culture treated cell culture plates or ELISA plates. Most of these experiments are related to ELIASA assay. If the background is more uniform, the experiment error would be reduced.

biousing 96-well plates have smoother upper and lower surface. This priority results in lower background error and helps users to get more reliable data in the experiments.

Another advantage of biousing 96-well plate is the bottom thickness. It is thinner than brand C and brand B. This helps to reduce edge effect because edge effect comes from the temperature difference between the marginal wells and the central wells [1]. If the bottom is thinner, the temperature will be quicker to get uniform，thus reducing edge effect.

Reference
1. BETINA KERSTIN LUNDHOLT, KURT M. SCUDDER, and LEN PAGLIARO, A Simple Technique for Reducing Edge Effect in Cell-Based Assays. Journal of Biomolecular Screening 2003:566-570